RPMI refers to Roswell Park Memorial Institute. RPMI-1640 medium was originally developed to culture human leukemic cells in suspnesion and as a monolayer. RPMI-1640 medium is unique from other media because it contains reducing glutathione and high concentrations of vitamins. Rpmi-1640 medium contains biotin, vitamin B12 and para-aminobenzoic acid, which are not found in Eagle’s Minimum Essential Medium or Dulbecco’s Modified Eagle Medium. In addition, inositol and choline are present in very high concentration. Hygia RPMI-1640 media preparations are available in with glucose or no glucose formulations, as well as with or without L-glutamine and sodium pyruvate. Hygia RPMI-1640 media include phenol red as a pH indicator. All available RPMI-1640 media products contain a sodium bicarbonate buffer with or without HEPES to maintain optimal culture pH.

RPMI-1640 medium has been found sutiable for a variety of mammalian cells, including Hela, Juukat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas. RPMI-1640 contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI-1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

All raw material components used for Hygia cell culture media products are screened through using strict quality control testing. Hygia cell culture media are manufactured at a cGMP-compliant facility using water for injection, filtered through final 0.1 μm sterile filtration and aseptic filled in a Hundred-stage FFU. The filter integrity tests are performed before and after filtration. Each lot of cell culture media are subjected to sterility test, pH, osmolality, endotoxin and cell culture test.

The major character of this RPMI-1640 medium is as follows:

1. With 11.1 mM Glucose, 2.05 mM GlutaPlus, phenol red.

2. Without sodium pyruvate and HEPES.

The complete formulation is available.

Storage and Handling Conditions:

Transport at room temperature; Store at 2-8℃, valid for 12 months.

Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation during use to avoid contamination;；

2. In order to maintain the best use effect, do not freeze and thaw repeatedly.

For Research Use Only!