Biologix Wash Buffers for IHC, Western Blot, ELISA, Southern blot, Northern blot, and other immunoassay assays are antibody diluents or non-specific antibody washes containing PBS, PBST, TBS, TBST, and SSC buffers.
Guide
1. TBS system buffer is available for phosphorylated antibody detection or repeated use after antibody dilution. Phosphate buffer is not suitable for the Alkaline Phosphatase (AP) assay system.
2. PBST & TBST are PBS & TBS buffers containing Tween -20, respectively, which help in the elution of non-specific proteins.
3. SSC has been autoclaved and can be used for denaturation and cleaning in a variety of hybridization experiments, while it is also the most standard blotting and hybridization treatment solution in molecular biology.
Product Nature
| PBS-510 | PH | 7.4±0.1 (Dilute to 1×) |
| Phenol red indicator | No phenol red | |
| Sterile | Sterile | |
| Osmotic pressure | 270 - 315 mOsm/kg (Dilute to 1×) | |
| Endotoxin | / |
| PBST-500 | PH | 7.4±0.1 |
| Phenol red indicator | No phenol red | |
| Sterile | Non-sterile | |
| Osmotic pressure | / | |
| Endotoxin | / |
| TBST-500 | PH | 7.5±0.1 |
| Phenol red indicator | No phenol red | |
| Sterile | Non-sterile | |
| Osmotic pressure | / | |
| Endotoxin | / |
| TBS-500 | PH | 7.6±0.1 |
| Phenol red indicator | No phenol red | |
| Sterile | Non-sterile | |
| Osmotic pressure | / | |
| Endotoxin | / |
| SSC-500 | PH | 7.0±0.1 |
| Phenol red indicator | No phenol red | |
| Sterile | Non-sterile | |
| Osmotic pressure | / | |
| Endotoxin | / |
| Cat.# | Product Name | Formula | Package |
| PBS-510 | PBS pH7.4 buffer(10×) | 27 mM KCl, 1.37 M NaCl, 100 mM Na2HPO4,18 mM KH2PO4 | 500mL/ Bottle, 10 Bottles/Case |
| PBST-500 | PBST pH7.4 buffer(1×) | 2.6 mM KCl, 136 mM NaCl, 8 mM Na2HPO4,2 mM KH2PO4, 0.05% (V/V)Tween-20 | 500mL/ Bottle, 10 Bottles/Case |
| TBST-500 | TBST pH7.5 buffer(1×) | 150 mM NaCl, 10 mM Tris,0.05% (V/V) Tween-20 | 500mL/ Bottle, 10 Bottles/Case |
| TBS-500 | TBS pH7.5 buffer(1×) | 150mM NaCl, 10mM Tris | 500mL/ Bottle, 10 Bottles/Case |
| SSC-500 | SSC pH7.0 buffer(1×) | 150mM NaCl, 15mM Sodium Citrate | 500mL/ Bottle, 10 Bottles/Case |
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