This product is a dry powder type of LB broth culture medium. The product specification is 250g per bottle. It is used for general bacterial culture, especially for the preservation and culture of E.coli in molecular biology test.
Instructions for use:
1. Weigh out 25g of this product
2. Add distilled water or deionized water at 1 L
3. Stir and heat to boiling until completely dissolved
4. Aliquot into test tubes or triangular flasks
5.Sterilize by autoclaving at 121℃ for 15 minutes before use
When used for Escherichia coli culture tests, the growth conditions of the bacteria are basically the same. Taking Escherichia coli DH5 a (containing pUC19 plasmid) and Escherichia coli ATCC25922 as examples, the actual test results are shown in the figure.
Ingredients :
| LBB-250 | Formula (1L) | Amount (g) |
| Tryptone | 10.0 | |
| Sodium Chloride | 10.0 | |
| Yeast Paste | 5.0 | |
| Final pH (25℃ ) | 7.0±0.2 |
* Note: The reference of the formula ingredients is Molecular Cloning: A Laboratory Manual Vol.3, p.1513.
| LBB-251 | Component | Content (g/L) |
| Tryptone | 10.0 | |
| Yeast extract | 5.0 | |
| Sodium Chloride | 5.0 | |
| Final pH (25℃ ) | 7.0±0.2 |
Storage: 10-30℃ , stable for 3 years before unsealing
Quality Control:
Quality Control Strains with Code | Index | Cultivation Conditions | Quality Control Evaluation Criteria |
Escherichia coli ATCC25922 | Growth rate | Culture at 36±1℃ for 18~24 h | PR ≥ 0.7, gray-white colonies |
| Cat.# | Product Name | Description | Qty |
| LBB-250 | LB Broth | LB Broth culture medium,bottled powder | 250g/ Bottle, 10 Bottles/Case |
| LBB-251 | LB Broth | LB Broth (Lennox), Low-Salt Version | 250g/Bottle, 10 Bottles/Case |
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