DMEM/F-12 is a 1:1 mixture of DMEM and Ham’s F-12, and it combines DMEM’s high concentrations of glucose, amino acids, and vitamins with F-12’s wide variety of components. DMEM/F-12 is widely used for supporting the growth of many different mammalian cells, including MDCK, glial cells, fibroblasts, human endothelia cells, and rat fibroblasts. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).